A Chinese Medicine, Huajie Xiaoliu Formula, Enhances the Anti-Colon Cancer Effect of Tegafur, Gimeracil, and Oteracil Potassium Capsules Through Bcl-2/Bax Signaling Pathway
نویسندگان
چکیده
Research Objective This study was aimed to determine the main ingredients of Huajie Xiaoliu Formula (HJF) and investigate anti-colon cancer effect its combination with Tegafur, Gimeracil, Oteracil Potassium Capsules (S-1) in vivo as well preliminarily explore possible mechanism. Materials Methods The chemical composition HJF determined by ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). murine colon carcinoma cell (CT 26.WT) subcutaneous xenograft model mice established evaluate HJF. Evaluation pathological changes tumor tissue evaluated hematoxylin–eosin staining. Enzyme-linked immunosorbent assay (ELISA) used measure expressions interleukin-1β (IL-1β), inflammatory cytokines interleukin (IL-6), necrosis factor-α (TNF-α) serum. Immunohistochemistry B lymphoma-2 (Bcl-2), Bax, Ki67 protein expression tissue. Results Twenty chemicals were identified UPLC-QTOF-MS. In experiments showed that has obvious inhibitory effects on mice. ELISA is able increase body weight, inhibit growth, reduce serum levels IL-1β IL-6 along TNF-α. results demonstrated can significantly Bcl-2, transplant tumors. Furthermore, S-1 a more significant antitumor than any other group vivo. Conclusions effective CT 26.WT inhibiting tumorigenesis, metastasis, angiogenesis through Bcl-2/Bax signaling pathway, resulting anti-colorectal effects. provides strong theoretical basis for clinical use also further research.
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ژورنال
عنوان ژورنال: Natural Product Communications
سال: 2023
ISSN: ['1555-9475', '1934-578X']
DOI: https://doi.org/10.1177/1934578x231185201